個人簡介

李錦铨，77779193永利官网教授，長期緻力于食源性病原菌的噬菌體防控機制研究和噬菌體暗物質的發掘。率先建立了沙門氏菌、大腸杆菌、金黃色葡萄球菌、副溶血弧菌、氣單胞菌等重要食源性病原菌的噬菌體庫，為實現我國噬菌體産業自主生産積累資源，相關技術可應用于食品安全、也可用于替代飼料和養殖中的抗菌物質。通過組學方法高通量分離和篩選到噬菌體裂解酶、噬菌肽和多糖解聚酶等噬菌體産物，可作為綠色新型的抗菌分子可替代醫用抗生素。運用無痕基因編輯技術構建噬菌體生産底盤細胞。此外，發掘新型噬菌體、益生噬菌體以及噬菌體與機體互作新機制。曾在中國食品學報、食品科學、EMBO Molecular Medicine、FEMS microbiology reviews、Journal of Controlled Release、The Journal of Infectious Diseases、Food control、International Journal of Food Microbiology、Virulence、Infection and immunity等期刊發表論文40餘篇。主持國家自然科學基金4項、湖北省自科基金中美合作專項1項、湖北省自然科學基金1項，參加國家重點研發計劃1項，授權專利10餘項。任《77779193永利官网學報》青年編委， MDPI-antibiotics和Frontiers in Microbiology主題編輯，《畜牧獸醫學報》、《食品科學》、《中國漁業質量與标準》、《食品安全質量檢測學報》、Microbiology Spectrum、Veterinary Microbiology、BMC Genomics、Infection and Immunity和International Journal of Food Microbiology等期刊審稿人。

教育經曆

2005-2009 77779193永利官网/生物技術/學士
2009-2013 77779193永利官网/預防獸醫/博士，導師：陳煥春院士
2011-2013 Montana State University/感染與免疫/中美聯培博士

工作經曆

2021-至今77779193永利官网教授
2021-至今上海噬菌體與耐藥研究所兼職PI
2020-至今 77779193永利官网/交叉科學研究院研究員
2020-至今洛克菲勒大學/噬菌體與傳染病客座研究員
2018-2020 洛克菲勒大學/傳染病博士後合作導師：Vincent A. Fischetti教授
2013-2021 77779193永利官网講師、副教授

研究領域

1. 耐藥食源性病原菌噬菌體資源庫和綠色生産工藝（發酵工藝，納米包埋技術）
2. 基于組學技術高通量篩選新型噬菌體裂解酶/噬菌肽
3. 新功能噬菌體和益生噬菌體的發掘（編輯腸道菌群、食品安全防控、腫瘤微環境、代謝性疾病）
4. 食品安全快速檢測技術

科研項目

1. 人獸共患病與公共衛生，2024.01-2026.12，國家自然科學基金優秀青年科學基金項目，主持
2.湖北省傑出青年科學基金，2023至2026，主持
3. 農業農村部農業科研傑出人才培養計劃，2021.09至2022.09，主持
4. 高效裂解耐藥大腸杆菌的非透膜劑依賴型噬菌體裂解酶作用機制研究， 2021.1-2024.12，國家自然科學基金面上項目，主持
5. 噬菌體多糖解聚酶介導的腸炎沙門氏菌生物被膜消除機制， 2018.1-2021.12，國家自然科學基金面上項目，主持
6.“十四五”國家重點研發計劃“豬細菌性疫病新型疫苗創制”項目子課題, 2022.11-2025.12,國家重點研發計劃,主持
7. 豬鍊球菌2型内皮素轉化酶的緻病機制研究，國家自然科學基金青年項目，2016.1-2018.12，主持
8. 耐藥梭菌噬菌體高效裂解酶的發掘和作用機制研究，2019.1-2020.12，湖北省技術創新專項，主持
9. 湖北地區小龍蝦中副溶血弧菌污染狀況和污染機制研究，2015.1-2016.12，湖北省自然科學基金，主持
10. 重要食源性緻病菌耐藥機制及傳播規律研究，2018.1-2021.12，國家重點研發計劃，研究骨幹

成果獎勵

其它

代表成果：
A分子流行病學和噬菌體配伍技術
A1. Geng Zou, Lijun He, Jing Rao, Zhiyong Song, Hu Du, Runze Li, Wenjing Wang, Yang Zhou, Lu Liang, Huanchun Chen, Jinquan Li*. Improving the safety and efficacy of phage therapy from the perspective of phage-mammal interactions. FEMS microbiology reviews. 2023 Jul 5;47(4): fuad042.
A2. Xingxing Dong#, Yanjie Chao#, Yang Zhou, Rui Zhou, Wei Zhang, Vincent A. Fischetti, Xiaohong Wang, Ye Feng*, Jinquan Li*. The global emergence of a novel Streptococcus suis clade associated with human infections. EMBO Molecular Medicine, 2021, e13810（本研究被Nature選為全球研究熱點Research Highlights并在主頁專題報道）
A3. Yang Zhou, Xingxing Dong, Zhiwei Li, Geng Zou, Li Lin, Xiaohong Wang, Huanchun Chen, Robin B. Gasser, Jinquan Li*. Predominance of Streptococcus suis ST1 and ST7 in human cases in China, and detection of a novel sequence type, ST658. Virulence, 2017, 8(6):1031-1035.
A4. Xingxing Dong, Zhi Li, Xiaohong Wang, Min Zhou, Li Lin, Yang Zhou, Jinquan Li*. Characteristics of Vibrio parahaemolyticus isolates obtained from crayfish (Procambarus clarkii) in freshwater. International Journal of Food Microbiology, 2016:132-138.
A5. Xingxing Dong, Xiaohong Wang, Xingchun Chen, Zhiyun Yan, Jing Cheng, Liangliang Gao, Yuan Liu, Jinquan Li*. Genetic Diversity and Virulence Potential of Staphylococcus aureus Isolated from Crayfish (Procambarus clarkii). Current Microbiology, 2017, 74(1):28-33.
B 噬菌體庫和噬菌體/裂解酶食品安全控制技術
B1. Geng Zou, Libère Ndayishimiye, Lingxiang Xin, Manshan Cai, Longjian Zhang, Jie Li , Zhiyong Song, Renwei Wu, Yang Zhou, Yuanguo Shi, Yingwang Ye, Rui Zhou, Jinquan Li*.Application of a novel phage LPCS28 for biological control of Cronobacter sakazakii in milk and reconstituted powdered infant formula. Food Research International, 2023,172: 113214.
B2. Chad W Euler, Assaf Raz, Anaise Hernandez, Anna Serrano, Siyue Xu, Martin Andersson, Geng Zou, Yue Zhang, Vincent A Fischetti, Jinquan Li*. PlyKp104, a Novel Phage Lysin for the Treatment of Klebsiella pneumoniae, Pseudomonas aeruginosa, and Other Gram-Negative ESKAPE Pathogens. Antimicrobial Agents and Chemotherapy, 2023,17;67(5): e0151922.
B3.Yue Zhang, Geng Zou, Md Sharifull Islam, Kun Liu, Suqiang Xue, Zhiyong Song, Yingwang Ye, Yang Zhou, Yuanguo Shi, Shaozhong Wei, Rui Zhou, Huanchun Chen, Jinquan Li*. Combine thermal processing with polyvalent phage LPEK22 to prevent the Escherichia coli and Salmonella enterica contamination in food. Food Research International, 2022, 165:112454.
B4. Lin Teng, Geng Zou, Yang Zhou, Jie Li, Zhiyong Song, Xingxing Dong, Zhengxin Ma, Zhijie Zheng, Huanchun Chen,Li Jinquan*.Phage controlling method against novel freshwater-derived Vibrio parahaemolyticus in ready-to-eat crayfish (Procambarus clarkii). Food Research International, 2022,162:111986.
B5. Yating Guo#, Jie Li#, Md.Sharifull Islam, Ting Yan, Yang Zhou, Lu Liang, Ian F.Connerton, Kai Deng, Jinquan Li*. Application of a novel phage vB_SalS-LPSTLL for biological control of Salmonella in foods. Food Research International, 2021, 147:110492.
B6. Siyue Xu, Edmondo Campisi*, Jinquan Li*, Vincent A. Fischetti. Decontamination of Escherichia coli O157:H7 on Fresh Romaine Lettuce Using a Novel Bacteriophage Lysin. International Journal of Food Microbiology, 2021, 341:109068.
B7. Sharifull Islam, Md, Xiangwei Yang, Chad W. Euler, Xueqing Han, Jiahui Liu, Isrfil Hossen, Md, Yang Zhou, Jinquan Li*. Application of a novel phage ZPAH7 for controlling multidrug-resistant Aeromonas hydrophila on lettuce and reducing biofilms. Food Control, 2021, 122 :107785
B8. Md. Sharifull Islam, Yang Zhou, Lu Liang, Ishatur Nime, Ting Yan, Stephan P. Willias, Md. Zakaria Mia, Weicheng Bei, Ian F. Connerton, Vincent A. Fischetti, Jinquan Li*. Application of a Broad Range Lytic Phage LPST94 for Biological Control of Salmonella in Foods. Microorganisms, 2020, 8(2):247.
B9. Ting Yan, Lu Liang, Ping Yin, Yang Zhou, Ashraf Mahdy Sharoba, Qun Lu, Xingxing Dong, Kun Liu, Ian F. Connerton, Jinquan Li*. Application of a Novel Phage LPSEYT for Biological Control of Salmonella in Foods. Microorganisms, 2020, 8(3):400.
B10. Md. Sharifull Islam, Yang Zhou, Lu Liang, Ishatur Nime, Kun Liu, Ting Yan, Xiaohong Wang, Jinquan Li*. Application of a Phage Cocktail for Control of Salmonella in Foods and Reducing Biofilms. Viruses, 2019, 11(9): 841.
B11. Chenxi Huang, Safiullah M. Virk, Jianchun Shi, Yang Zhou, Stephan P. Willias, Mohamed K. Morsy, Hazem E. Abdelnabby, Jie Liu, Xiaohong Wang*, Jinquan Li*. Isolation, Characterization, and Application of Bacteriophage LPSE1 Against Salmonella enterica in Ready to Eat (RTE) Foods. Frontiers in Microbiology, 2018, 9:1046.
B12. 一種人工合成的噬菌肽SEYT1及其應用. CN 114907451 B.發明專利.2023.08.中國.1/8
B13. 一株能夠裂解阪崎腸杆菌的廣譜噬菌體LPCS28及應用. CN 115011564 B.發明專利.2023.06.中國.1/5.
B14. 一種源于沙門氏菌噬菌體基因編碼的蛋白在作為革蘭氏陰性菌裂解酶中的應用. CN112175928A. 發明專利. 2022.09.中國.1/3.
B15. 一種腸炎沙門氏菌噬菌體LPSE28及其在食品中的應用. CN108546685B. 發明專利. 2021.01. 中國. 1/9.
B16. 一種沙門氏菌噬菌體LPSE34及其在食品中的應用. CN108588037B. 發明專利. 2021.01.中國. 1/9.
B17. 一種主效成分為噬菌體LPEE17和LPEK22的混合制劑及應用. CN110317793B. 發明專利. 2020.06. 中國. 1/4.
B18. 沙門氏菌噬菌體和噬菌體抗菌組合物及其應用. CN106497888B. 發明專利. 2018.06. 中國. 1/8.
B19. 一種寬譜沙門氏菌噬菌體LPSTLL及應用. CN109825479B. 發明專利. 2019.02. 中國. 2/7.
B20. 呂陽（本科生），尹平，Khairy Morsy MOHAMED，高磊，袁超，周洋，邱甯，鄒璐瑤，李志，Abdelnabby HAZEM，王小紅，李錦铨*. 噬菌體在食品安全控制中的研究進展, 食品科學, 2018,(15):240-246.
B21. 郭浩然（本科生），許菁彥，周洋，李子齊，邱熠鑫，魏麟，董星星，李錦铨*. 噬菌體分布的廣泛性以及口服安全性，中國食品學報，2021, 21(02):349-357.
C噬菌體綠色生産工藝（基因工程減毒底盤細胞）
C1. Zhiwei Li, Peixi Chang, Jiali Xu, Chen Tan, Xiaohong Wang, Weicheng Bei, Jinquan Li*. A Streptococcus suis Live Vaccine Suppresses Streptococcal Toxic Shock-Like Syndrome and Provides Sequence Type Independent Protection. The Journal of Infectious Diseases, 2019, 219(3): 448-458.
C2. Jinquan Li﹟, Chen Tan﹟, Yang Zhou, Shulin Fu, Linlin Hu, Jin Hu, Huanchun Chen, Weicheng Bei*.The two-component regulatory system CiaRH contributes to the virulence of Streptococcus suis 2. Veterinary Microbiology, 2011, 148(1):99-104.
C3. Ying Zhou, Ruonan Nie, Xiaoyue Liu, Jinghui Kong, Xiaohong Wang, Jinquan Li*. GntR is involved in the expression of virulence in strain Streptococcus suis P1/7. FEMS Microbiology Letters, 2018, 365(14).
C4. 一種無抗生素标記的嗜水氣單胞菌減毒菌及應用.CN105886429B.發明專利. 2019.04.中國.2/7.
D食品安全檢測技術和納米包埋技術
D1. Yufan Yang, Hu Du, Geng Zou, Zhiyong Song, Yang Zhou, Hao Li, Chen Tan, Huanchun Chen, Vincent A Fischetti, Jinquan Li*. Encapsulation and delivery of phage as a novel method for gut flora manipulation in situ: A review. Journal of Controlled Release, 2023,353: 634-649.
D2. Yu Jin, Ke Liu, Guangqiang Li, Chengfei Li, Zhidong Xiao, Chao Yuan*, Jinquan Li*. In situ reduction triggers the highly sensitive detection of pesticide by classic gold nanoparticle and quantum dots nanocomposite. Analytica Chimica Acta, 2021, 1172:338679
D3. Responsive metal–organic framework nanocarrier delivery system: An effective solution against bacterial infection. Yi Liu, Jinmei Wu, Weihui Li, Jinquan Li, Heyou Han, Zhiyong Song*. Coordination Chemistry Reviews 496, (2023) 215431
D4. Shaping Huang, Liping Song, Zhidong Xiao*, Yue Hu, Meiwen Peng, Jinquan Li, Xinsheng Zheng, Bin Wu, Chao Yuan*. Graphene quantum dot-decorated mesoporous silica nanoparticles for high aspirin loading capacity and its pH-triggered release. Analytical Methods, 2016, 8(12)
D5. Ahmed Mohamed, Xuemeng Li, Jinquan Li, Chuangye Lin, Abdullah M. Asiri, Hadi M. Marwani, Suhua Wang, Zhidong Xiao*, Bin Li*, Chao Yuan*. Single microbead-based fluorescence "turn on" detection of biothiols by flow cytometry. Talanta, 2019, 195
D6. 魏麟（本科生），朱方莉，周洋，Khairy Morsy MOHAMED，袁超，董星星，王小紅，李錦铨*. 噬菌體在檢測食源性病原菌中的應用研究進展. 食品科學, 2018，(1):314-322.
D7. 一種雞常見食源性細菌四重熒光PCR檢測試劑盒及使用方法. CN103436626B.發明專利. 2015.09.中國.8/9.
D8. 一種豬鍊球菌2型的SS2-LAMP檢測試劑盒及應用.CN104404132B.發明專利.2016.02.中國.1/5.
D9. 一種區分豬鍊球菌爆發菌株和散發菌株的檢測引物和應用.CN106868143B.發明專利.2018.10.中國.1/10.
E 病原菌免疫逃逸機制和疫苗研發
E1. Feng Liu#, Jinquan Li#, Kang Yan, Huan Li, Chengfeng Sun, Shuo Zhang, Fangyan Yuan, Xiangru Wang, Chen Tan, Huanchun Chen, Weicheng Bei*, Binding of Fibronectin to SsPepO Facilitates the Development of Streptococcus suis Meningitis, The Journal of Infectious Diseases, 2018, 217(6):973-982.
E2. Jinquan Li, Guanghui Liu,Wenchao Feng,Yang Zhou, Mengyao Liu, James A. Wileya and Benfang Lei*. Neutrophils Select Hypervirulent CovRS Mutants of M1T1 Group A Streptococcus during Subcutaneous Infection of Mice. Infection and Immunity, 2014, 82(4):1579-90.
E3. Jinquan Li, Hui Zhu, Wenchao Feng, Mengyao Liu, Yingli Song, Xiaolan Zhang, Yang Zhou, Weicheng Bei, Benfang Lei*. Regulation of Inhibition of Neutrophil Infiltration by Two-Component Regulatory System CovRS in Subcutaneous Mouse Infection with Group A Streptococcus. Infection and Immunity, 2013, 81(3): 974-983.
E4. Yachan Ji﹟, Jinquan Li﹟, Zhendong Qin, Aihua Li, Zemao Gua, Xiaoling Liu, Li Lin, Yang Zhou*. Contribution of nuclease to the pathogenesis of Aeromonas hydrophila. Virulence, 2015, 6(5):515-22. doi: 10.1080/21505594.2015.1049806.
E5. Liping Feng, Xiaona Niu, Wen Mei, Weitian Li, Yuan Liu, Stephan P. Willias, ChaoYuan, Weicheng Bei, Xiaohong Wang, Jinquan Li*. Immunogenicity and protective capacity of EF-Tu and FtsZ of Streptococcus suis serotype 2 against lethal infection. Vaccine, 2018, 36(19):2581-2588.
E6. 一種豬鍊球菌2型10基因缺失株及應用. CN106085936B.發明專利.2019.02.中國.1/6.
E7. 一種豬鍊球菌2型五基因缺失株及應用.CN105316252B.發明專利.2018.08.中國.1/7.
E8. 一種高緻病性嗜水氣單胞菌及應用.CN105695372B.發明專利.2018.05.中國.2/7.